Nickolaos Nikiforos Giakoumakis

 

Nickolaos Nikiforos Giakoumakis, PhD

Laboratory of General Biology

School of Medicine

University of Patras

Rio, Patras, 26505, Greece


email: nickngiak[at]gmail[dot]com

Education: 

  • 2012 - May 2017: PhD student, Laboratory of General Biology, Medical School, University of Patras
  • 2009 - 2012: MSc, Interdepartmental Master's Program Informatics for Life Sciences specializing in Bioinformatics.
  • 2003 - 2009: Diploma, Biology Department,  University of Crete, with expertise in Molecular Biology and Biotechnology.

Reaserch Interests: 

  • Image analysis, functional imaging, live cell Imaging, FRAP, FRET, Quantitative Biology.

Projects:

  • easyFRAP
  • Cell tracking and Image Quantification
  • DNA damage response pathways in different cancer cell lines
  • Licensing factors in DDR pathways

Honours & Awards:

  • Best oral presentation award at the 67th Panhellenic Conference of the HSBMB at Ioannina 25-27 November 2016.
  • Eurobioimaging proof-of-Concept-studies fellowship for conducting experiments at the Advanced Digital Microscopy Facility of the Institute of Research in Biomedicine of Barcelona April 2012- May 2012.
  • Erasmus Placements Scholarship for Master Studies for conducting experiments at theAdvanced Digital Microscopy Facility of the Institute of Research in Biomedicine of Barcelona June 2012- August 2012.
  • EMBL Corporate Partnership Registration Free Fellowship for the participation of EMBL Advanced Course: Advanced Imaging Techniques. Heidelberg - 2012.
  • EMBL Visiting Predoctoral Fellow at the Advanced Light Microscopy Facility, under the supervision of dr. Pepperkok, November 2014-February 2015, Heidelberg.

Oral Presentations/Posters:

          Oral Presentations

  • M.A. Rapsomaniki, N.N. Giakoumakis, P. Kotsantis, I.E. Symeonidou & Z. Lygerou.”A user-friendly FRAP analysis tool to assist FRAP data normalization.” 17-19 October 2010 Hellenic Meeting of the Computational Biology and Bioinformatics Association, Alexandroupolis, Greece.
  • N.N. Giakoumakis, Practical Course Instructor at “Views into Nuclear Function, A SEEDRUG workshop” 11-13 September, Patras, Greece.
  • N.N. Giakoumakis and M.A. Rapsomaniki “Fluorescence Recovery After Photobleaching (FRAP) in the study of protein kinetics: data analysis, modelling and parameter inference.” “13-15 October 2014. Spanish Advanced Optical Microscopy Network (REMOA 2014), Madrid, Spain.

  • N.N. Giakoumakis, S. Maxouri, S. Taraviras, Z. Lugerou “A Semi-Automatic Quantification method for observing the heterogeneity in re-replication patterns in fission yeast”. 17 December 2015. Center for Molecular and Cellular Imaging of EMBL, Heidelberg, Germany.

          Selected Posters

  • N.N. Giakoumakis, T. Giavridis, P. Kotsantis, S. Taraviras & Z. Lygerou.. “Cell type specific regulation of Cdt1 and p21 proteolysis in cancer cells lines upon ultraviolet microirradiation”. DNA damage in Disease, Barcelona 28-30 May 2012, Spain.
  • N.N. Giakoumakis, J. Colombelli, P. Kotsantis, S. Taraviras, Z. Lygerou. “Quantifying the post DNA-damage protein kinetics and interactions of the factor Cdt1 and its recruitment at the site of DNA damage”/ EMBL Master Course- Bioimage Data Analysis 13-17 May 2013, Heidelberg, Germany.
  • N.N. Giakoumakis, A. Panagopoulos, S. Taraviras, Z. Lygerou “Cdt1 complex formation at sites of DNA damage: assessing effects of post-translational modification by functional live-cell imaging”, Views into Nuclear Function, A SEEDRUG workshop” 11-13 September 2014, Patras, Greece.
  • N.N. Giakoumakis, J. Colombelli, S. Taraviras , Z. Lygerou. “Differential recruitment dynamics of polymerases following DNA irradiation in the absence of Cdt1” EMBO conference The DNA damage response in cell physiology and disease. Sounio, 5-9 October, Greece.
  • N.N. Giakoumakis, A. Panagopoulos, H. Nishitani, S.Taraviras Z. Lygerou. “Recruitment kinetic properties of Cdt2 post localised DNA” EMBO conference The DNA damage response in cell physiology and disease. Sounio, 5-9 October, Greece.

Publications (peer-reviewed):

  1. P. Galanos, G. Pappas, A. Polyzos, A. Kotsinas, I. Svolaki, N.N. Giakoumakis, C. Glytsou, I. S. Pateras, U. Swain; V. Souliotis, A. G. Georgakilas, N. Geacintov, L. Scorrano, C. Lukas, J. Lukas; Z. Livneh, Z. Lygerou, D. Chowdhury, C. S. Sørensen, J. Bartek, V. Gorgoulis (2018) Mutational signatures reveal the role of RAD52 in p53-independent p21 driven genomic instability. Genome Biology,in press.
  2. Giakoumakis ,N.N. Rapsomaniki, M. A. and Z. Lygerou (2017) Analysis of Protein Kinetics Using    Fluorescence Recovery After Photobleaching (FRAP). Methods Mol. Biol, 1563: 243-267.1.
  3. Kourti, Ikonomou, Giakoumakis, Rapsomaniki, Landegren, Siniossoglou, Lygerou, Simos,Mylonbis (2015)    CK1-δ restrains lipin-1 induction, lipid droplet formation and cell proliferation under hypoxia by reducing HIF-1α/ARNT complex formation, Cellular Signalling 1873-3913. doi 10.1016.
  4. Rapsomaniki MA, Cinquemani E, Giakoumakis NN, Kotsantis P, Lygeros J, Lygerou Z. (2015) Inference           of protein kinetics by stochastic modeling and simulation of Fluorescence Recovery After Photobleaching           experiments. Bioinformatics,1;31(3):355-62.
  5. Rapsomaniki MA, Kotsantis P, Symeonidou IE, Giakoumakis NN, Taraviras S and Lygerou Z (2012).     easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of Fluorescence Recovery After Photobleaching data. Bioinformatics. 28(13):1800-1.